Television
The Royal Flush Gang appears in The Super Powers Team: Galactic Guardians episode "The Wild Cards", with King voiced by Eugene Williams, Queen voiced by Arlene Golonka, Jack voiced by Jerry Houser, and Ten voiced by Lynne Moody. This version of the group were originally thieves until they were recruited by the Joker under the alias of Ace.
Three incarnations of the Royal Flush Gang appear in series set in the DC Animated Universe (DCAU).
The first incarnation appears in Batman Beyond, with King voiced by George Lazenby, Queen voiced by Amanda Donohoe in the first season and Sarah Douglas in all subsequent appearances, their son Jack voiced by Scott Cleverdon in the first season and Nicholas Guest in the third season, and their daughter Melanie Walker / Ten voiced by Olivia d'Abo, while Ace is silent. This version of the group is a multi-generational crime family who use high-tech, playing card-themed weapons and an android serving as Ace. Additionally, they previously encountered Bruce Wayne as the original Batman, who nearly succeeded in disbanding the gang's predecessors. Throughout their appearances, the gang battle Batman (Terry McGinnis), who falls in love with Melanie and eventually motivates her to leave the group. Following this, the gang struggle to maintain their power and work with their employer Paxton Powers to assassinate Wayne, only to be thwarted by Batman. In the aftermath, Ace is destroyed, King and Queen are arrested, the former is revealed to have been pursuing an affair with Powers's secretary due to an inferiority complex stemming from being compared unfavorably to his late predecessor and father-in-law, and Melanie bails Jack out of jail and helps him get a job at the restaurant she works at.
The second incarnation appears in the Justice League episode "Wild Cards", with King voiced by Scott Menville, Queen by Tara Strong, Jack by Greg Cipes, Ten by Khary Payton, and Ace by Hynden Walch. This version of the group are Project Cadmus-trained, metahuman teenagers, with King possessing pyrokinesis, Queen metallokinesis, Jack elasticity, Ten superhuman strength, and Ace psionic abilities. The Joker finds them, turns them into his Royal Flush Gang, and pits them against the Justice League on live television while he secretly uses Ace to drive millions of viewers insane. After the Leaguers capture most of the gang, Batman discovers the Joker kept a collar that Ace's government handlers used to control her as insurance against her. An enraged Ace subsequently puts him in a catatonic state before disappearing.
The third incarnation appears in a flashback in the Justice League Unlimited episode "Epilogue", with Ace voiced again by Hynden Walch while the rest of the gang are silent. This version of the group was formed by a lonely and depressed Ace, who developed reality-warping powers. She used them to turn four random people into a new Royal Flush Gang to play with her, but they chose to abandon her and turned to crime. Their powers are puns based partially on their names: King is a massive head with small limbs resembling Egg Fu and Marvel Comics character MODOK who possesses a flying throne and eye beams; Queen was transformed from a man and given enhanced strength; Jack is a samurai transformed from a black man; and Ten is a woman with extendable whip-like hair evocative of Bo Derek's character from 10. While the Justice League battled the empowered criminals, Cadmus member Amanda Waller revealed that Ace was dying due to an aneurysm and a possible psychic backlash caused by her death could kill millions. Batman volunteered to kill her before that could happen and confronted her, but Ace knew he had no intent to do so. After briefly explaining her backstory to him, she asked him to keep her company until she died. Batman accepted. Ace's peaceful death harmed no one, and restored the remaining gang members to normal.
The Royal Flush Gang appear in Batman: The Brave and the Bold, with Ace voiced by Diedrich Bader and Jack by Edoardo Ballerini while King and Queen are silent. This version of the gang are Old West bandits.
The Royal Flush Gang appear in the DC Super Hero Girls episode "#AwesomeAuntAntiope".
Three incarnations of the Royal Flush Gang appear in series set in the Arrowverse:
The first incarnation appears in the Arrow episode "Legacies", with Derek Reston / King portrayed by Currie Graham, Kyle Reston / Ace by Kyle Schmid, Teddy Reston / Jack by Tom Stevens, and Derek's unnamed wife / Queen by Sarah-Jane Redmond. This version of the group is a family of bank robbers who wear hockey masks marked with their respective playing card. Additionally, Derek previously worked for Queen Industries before its CEO Robert Queen outsourced jobs to China and the Reston family lost their home as a result. Feeling guilty, Oliver Queen tries to persuade Derek to right his own wrongs, but is forced to stop the Restons when they attempt to rob another bank. In the ensuing confrontation, Derek is fatally shot while the rest of the family are arrested. During Derek's final moments, Oliver reveals his secret identity to him to show the former that he always had his best interests at heart. Derek admits what he did was wrong and he should not have gotten his family involved before dying in Oliver's arms.
Two incarnations of the Royal Flush Gang appear in The Flash.
The first make a non-speaking cameo appearance in the episode "The Sound and the Fury", consisting of unnamed, masked motorcycle thieves King, Queen, and Ace who are swiftly apprehended by the Flash.
The second appear in the eighth season, consisting of metahumans Mona Taylor / Queen (portrayed by Agam Darshi) as the group's leader with psychic abilities; King (portrayed by Ryan Jefferson Booth) who has super-strength; Jake Fox / Jack (portrayed by Eston Fung) who can shoot lasers from his eyes; and Wanda Wayland / Ten (portrayed by Megan Peta Hill) who is an expert hand-to-hand combatant.
Sam Kurtis appears in Stargirl, portrayed by Geoff Stults. After disappearing in the pilot episode, he resurfaces in the episode "Shining Knight", ostensibly to reconnect with his daughter Courtney Whitmore, though he secretly attempts to steal her locket and sell it off for money. However, her stepfather Pat Dugan realizes Kurtis' true intentions and confronts him, telling him to never return.