Supporting
Trent Lane
Jane's older brother by five years, the second-youngest Lane sibling, and the only other sibling still permanently residing in the Lane household. He plays lead guitar in his band, Mystik Spyral. Daria's unspoken crush on Trent throughout the first three seasons is one of her few weaknesses.
Voiced by Alvaro J. Gonzalez.
Tom Sloane
A young man who serves as love interest to Jane and later to Daria. His parents are wealthy and he attends a nearby private school. He is, in Daria's words, "a little spoiled, a hair smug, and a trifle egotistical" but also a "smart, funny guy who's very caring and sensitive."
Voiced by Russell Hankin.
Brittany Taylor
Lawndale High's ditzy and bubbly head cheerleader; girlfriend to Kevin.
Voiced by Lisa Kathleen Collins, under the pseudonym Janie Mertz.
Kevin Thompson
The Lawndale High football team's quarterback and equally ditzy boyfriend to Brittany.
Voiced by Marc Thompson.
Michael "Mack" Jordan MacKenzie
Jodie's boyfriend and the Lawndale High football team's captain, as well as the only intelligent person on the team.
Voiced by Delon Ferdinand, Paul Williams, Kevin Daniels, and Amir Williams.
Jodie Landon
Lawndale High's class president and one of its few African-American students alongside her boyfriend Mack; she is one of Daria's few true friends other than Jane.
Voiced by Jessica Cydnee Jackson.
Charles "Upchuck" Ruttheimer, III
An obnoxious, sleazy flirt with curly red hair and freckles.
Voiced by Marc Thompson and Geoffrey Arend.
Andrea
The Lawndale High token goth who has a darkly nihilistic personality, moreso than Daria's. "Mart of Darkness" establishes that Andrea works at the local big box warehouse store and knows that Daria and Jane are infamous for making fun of the other students, which is why she initially doesn't help them out at the store. She relents when Daria and Jane promise never to tell anyone about her job or that they saw her.
Voiced by Susie Lewis Lynn and Lisa Kathleen Collins (credited under the name "Janie Mertz").
Sandi Griffin
The club president and most popular girl in school. She often tries to embarrass or sabotage Quinn due to jealousy.
Voiced by Lisa Kathleen Collins/Janie Mertz.
Stacy Rowe
The secretary of the club. Stacy is very insecure and neurotic and is the closest to Quinn.
Voiced by Jessica Zaino and Sarah Drew.
Tiffany Blum-Deckler
The sycophant of the club. She is known for her monotonous voice and lack of intelligence.
Voiced by Ashley Albert.
Joey, Jeffy, and Jamie
Three nearly interchangeable high-school students and members of the school's football team who constantly compete for Quinn's affection.
Joey was voiced by Geoffrey Arend and Steven Huppert.
Jeffy was voiced by Tim Novikoff.
Jamie was voiced by Marc Thompson.
Ms. Janet Barch
Science teacher. Due to her recent divorce, she hates all men, often taking out her frustration on Kevin, Mack, and Mr. DeMartino. However, she fell in love with Mr. O'Neill after they shared a tent (as seen on "The Daria Hunter"), leading to the running gag of Ms. Barch trying to make out with Mr. O'Neill every chance she gets (particularly during school functions), leading to her eventually marrying him (after Mr. DeMartino teaches Mr. O'Neill to stand up for himself and break up with her) during the series finale movie, "Is It College Yet?"
Voiced by Ashley Albert.
Mrs. Diane Bennett
Economics teacher who actually has great ideas and treats her students like human beings, both of which make her an extreme outlier at Lawndale High.
Voiced by Amy Bennett.
Ms. Claire Defoe
Art teacher who can be flighty but is intelligent about art.
Voiced by Nicole Carin and Danielle Carin.
Mr. Anthony DeMartino
History teacher. He often loses his temper due to his students' poor grasp of history. One of his eyes is bigger than the other, and noticeably gets larger the angrier he is. He is a lifelong bachelor and Vietnam War veteran. According to "The Daria Hunter" and "Antisocial Climbers," DeMartino grew up with a mother who cared more about the men in her life than taking care of her own son (DeMartino's story about spending time with the neighbors on the former episode, and his story about his former best friend marrying his mother on the latter episode). "The Daria Hunter" also revealed that DeMartino was sent to military school (which Jake -- a fellow military school student -- picks up on when DeMartino snatches his flask from him to heavily drink from) and that the neighbors he spent time with when his mother couldn't be bothered to raise him were "...strange, twisted people" and that Jake (whether or not he meant it, since the two of them were drunk) may have had those same neighbors.
Voiced by Marc Thompson.
Ms. Angela Li
School principal. Incredibly corrupt, her school safety policies often border on those of a police state and she will do anything to increase the budget and reputation of the school.
Voiced by Nora Laudani.
Mr. Timothy O'Neill
English teacher. He is often overly-sentimental and naïve when interacting with students.
Voiced by Marc Thompson.
Ms. Margaret Manson
School psychologist. She only has one speaking role, in which she declares Daria has self-esteem issues.
Voiced by Jessica Zaino.
Ms. Morris
Physical-education teacher, track team coach, and also the cheerleading coach. She is openly corrupt, turning physical-education class into covert cheerleading practice, and fudging grades for athletes.
Voiced by Katie Kingston.