Cast
Ben Stiller as Larry Daley, a night-shift security guard at the American Museum of Natural History in New York, Nick's father, and Erica's former husband.
Carla Gugino as Rebecca Hutman, a museum docent.
Dick Van Dyke as Cecil Fredericks, a veteran security guard.
Bill Cobbs as Reginald, a veteran security guard.
Mickey Rooney as Gus, a veteran security guard, who takes an instant dislike to Larry.
Robin Williams as Theodore Roosevelt, the wax sculpture of the 26th President of the United States dressed in his Rough Rider uniform, who befriends and mentors Larry.
Ricky Gervais as Dr. Leslie McPhee, the curator of the Museum of Natural History and Larry's boss.
Jake Cherry as Nick Daley, Larry's and Erica's son.
Owen Wilson as Jedediah (uncredited), a miniature cowboy figure.
Steve Coogan as Octavius, a miniature Roman general figure.
Rami Malek as the mummy of Ahkmenrah, a benevolent Egyptian pharaoh and the tablet's original owner, who becomes a fully living human at night. The film marks Malek's film debut.
Patrick Gallagher as a wax model of Attila the Hun, who antagonizes Larry at first before Larry appeals to his softer side.
Mizuo Peck as Sacagawea, the polyurethane model of the Lemhi Shoshone woman who guided Merriwether Lewis and William Clark, and later Theodore Roosevelt's girlfriend.
Pierfrancesco Favino as a bronze statue of Christopher Columbus, whose name Larry consistently forgets due to no label.
Kim Raver as Erica Daley, Larry's former wife and Nick's mother.
Paul Rudd as Don, Erica's fiancé.
Kerry van der Griend, Dan Rizzuto, Matthew Harrison, and Jody Racicot as wax models of Neanderthals.
Brad Garrett as the voice of the Easter Island Head.
Crystal the Monkey as Dexter, a stuffed Capuchin monkey who repeatedly torments Larry.
Martin Christopher as a wax model of Meriwether Lewis.
Martin Sims as a wax model of William Clark.
Randy Lee, Darryl Quon, Gerald Wong, and Paul Chih-Ping Cheng as wax models of Huns.
Charlie Murphy as the taxi-driver.
Anne Meara as Debbie.
Roger Lewis and Dave Hospes as miniature cowboys.
Jonathan Lee as a miniature Chinese railroad worker.
Jason Mckinnon as a miniature Irish railroad worker.
Jason Vaisvila, Cade Wagar, and Gary Sievers as wax models of Vikings.
Cory Martin as a Chinese terracotta soldier.
Trevor Addie as a miniature Roman.
Lloyd Adams as a soldier.
Matthew Walker as a politician.
