Speke is an English surname. Notable people with the surname include:
Anne Speke, Lady North or Anne North, Countess of Guilford (1740–1797), daughter of George Speke (died 1753)
George Speke (politician, died 1584) (c.1530–1584), English politician
George Speke (politician, died 1689) (1623–1689), English politician
George Speke (politician, died 1753) (c. 1686–1753), British politician, MP for Milborne Port, for Taunton, and for Wells
Sir George Speke, 2nd Baronet (1653–1683), English politician
Hugh Speke (1656–c.1724), English writer and agitator
Sir Hugh Speke, 1st Baronet (died 1661), English politician
John Hanning Speke (1827–1864), English explorer and army officer
John Speke (1442–1518), English sheriff
Mary Speke (c.1625–1697), English nonconformist patron and political activist