Recurring
Hilary Jardine as Susan Jackson (season 1; guest seasons 2–3, 5), Vanessa's neighbor and best friend up until The Rising, during which time she became a vampire.
Hannah Cheramy as Dylan (seasons 1–2, guest seasons 3, 5), the daughter of Vanessa.
Terry Chen as Brendan (season 1), the de facto leader of a group of survivors from Idaho.
Jennifer Copping as Quaid (season 1), a member of the Portland Human Resistance.
Ben Cotton as Campbell (season 1), a member of the Portland Human Resistance.
Gia Crovatin as Anastasia (seasons 1–2), an ancient vampire like her brother Dmitri.
John DeSantis as Gustov (season 1), a notable vampire from Julius' Brood.
Sarah Desjardins as Catherine (season 1), a member of a group of survivors from Idaho.
Christina Jastrzembska as Mama (season 1; guest seasons 2, 5), the mother of Julius, as well as a member of his Brood.
Macie Juiles as Callie (seasons 1–3; guest seasons 4–5), a member of a group of survivors from Idaho.
Avery Konrad as Cynthia (season 1), a young woman who survived The Rising.
Duncan Ollerenshaw as Dr. Sholomenko (season 1; guest season 2), a human doctor, who was forced into working for Dmitri and Rebecca in order to create day walking vampires with components from Vanessa's blood.
Rowland Pidlubny as Scab (seasons 1–4), a vampire servant from Julius' brood.
Ryan Robbins as Taka (season 1; guest season 2), a leader of the Portland Human Resistance.
Naika Toussaint as Sheema (season 1; guest season 2), the sister of Mohamad who is a member of the Portland Human Resistance.
Gwynyth Walsh as Magdalene (season 1), a vampire in allegiance with Julius who, as a human, was Micah's ex-wife.
Alison Wandzura as Nicole (season 1), a survivor held up in the Seattle Valley General Hospital until it was overrun with vampires.
Andrea Ware as Lucky (season 2), a member of the Portland Human Resistance.
Keith Arbuthnot as Abaddon (seasons 2–3), the first vampire elder and the oldest living vampire in existence.
Phil Burke as Mike (seasons 2–3), Chad's boyfriend and the co-leader of a group of mostly children refugees who hide out in the woods known as the "Johnsons".
Donny Lucas as Chad (seasons 2–4), Mike's boyfriend and the co-leader of a group of mostly children refugees who hide out in the woods known as 'The Johnsons'.
John Reardon as Dr. Bruce Harrison (season 2; guest season 3), a human performing experiments involving vampirism in a mountain fortress. He shares a mysterious connection to the Van Helsings.
Andee Frizzell as Abigail Van Helsing / The Boss (season 2; guest season 3), a scientist who works with Dr. Harrison, and claims to be Vanessa and Scarlett's mother.
Julie Lynn Mortensen as Lillian "Lily" Van Helsing (season 3; guest season 4), Vanessa's great-great-grandmother, a member of the Van Helsing family who lived in the late 19th to early 20th Century, and a vampire hunter.
Jennifer Spence as B'ah (season 3), the second vampire elder who fought against Lillian Van Helsing in the 19th century.
Michael Jonsson as Barry (season 3), one of a group of survivors whom Vanessa, Scarlett and Axel come across in San Francisco.
Danny Wattley as Dre (season 3), one of a group of survivors whom Vanessa, Scarlett and Axel come across in San Francisco.
Vanessa Walsh as Marybeth (season 3), the leader of a group of survivors whom Vanessa, Scarlett and Axel come across in San Francisco.
Kendall Cross as Frankie (season 3), a bartender in Denver who forms a relationship with Julius.
Anna Galvin as Avery (season 4; guest season 5), who works with Hansen at Fort Collins and is very interested in finding a "cure" for vampirism.
Richard Harmon as Max Borman (season 4), a sadistic prison warden and a smuggler.
Luvia Petersen as Sgt. Weathers (season 5), a member of the military who interrogates Ivory and Violet.
